Microscopy Techniques

Master the essential principles of optical imaging, sample preparation, and advanced microscopy. Capturing the unseen world with precision and clarity.

Brightfield Microscopy

Fundamentals of light microscopy including Kohler illumination, contrast enhancement methods, and objective lens selection.

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Fluorescence Imaging

Advanced techniques for visualizing specific structures using fluorophores, confocal scanning, and multi-channel image acquisition.

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Microscope Setup Checklist

Optics Cleaned Eyepieces and objectives checked for dust or oil. Lens paper and cleaning solution used if necessary.

Illumination Aligned Kohler illumination established. Field diaphragm centered and condenser focus adjusted for optimal contrast.

Sample Secured Slide properly seated in stage holder. Cover slip facing up and sample centered under the objective.

Certification Modules

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Optical Fundamentals

ID: OPT-101

Refraction, magnification, and resolution.

Sample Preparation

ID: PREP-200

Fixation, staining, and mounting techniques.

Digital Imaging

ID: IMG-305

Camera settings, processing, and analysis.

Microscopy FAQ

What is the resolution limit?

For standard optical microscopy, the diffraction limit is approximately 200nm. Super-resolution techniques can surpass this barrier.

When should I use immersion oil?

Immersion oil is required for high-numerical aperture objectives (typically 100x) to match the refractive index of glass and capture more light rays.

How do I clean objective lenses?

Use only lens tissue and approved optical cleaning solution. Never use facial tissue or kimwipes which can scratch the delicate coatings.